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. 2021 Mar 3;10:e65924. doi: 10.7554/eLife.65924

Figure 3. ICEBs1-containing cells delay sporulation in a frequency-dependent manner.

Figure 3.

ICEBs1-containing cells (JMJ593, black circles) were mixed with ICEBs1-cured cells (JMJ550, open squares) at an initial frequency of 0.01 and spotted onto biofilm growth medium (A) or sporulation medium (C). Mixtures with ICEBs1-containing cells at an initial frequency of 0.9 were also spotted onto biofilm medium (B) and sporulation medium (D). Biofilms and colonies were harvested at the indicated times to determine the fraction of CFUs derived from heat-resistant spores for both the ICEBs1-containing and the ICEBs1-cured cells. Boxes below each graph indicate the frequency of ICEBs1-containing CFUs at each timepoint. Data shown are the average from two populations (biological replicates) per timepoint with error bars indicating the standard deviation. A representative experiment is shown.

Figure 3—source data 1. Sporulation timing during competitions.
Raw counts and calculated frequencies of spore-derived CFUs and total CFUs measured at different times during competition of ICEBs1-containing and ICEBs1-cured cells.