Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Sost | National Center for Biotechnology Information | AAK13455 | |
| Gene (Homo sapiens) | Sost | National Center for Biotechnology Information | AAK16158.1 | |
| Gene (Rattus norvegicus) | Sost | National Center for Biotechnology Information | EDM06161.1 | |
| Gene (Bos Taurus) | Sost | National Center for Biotechnology Information | NP_001159986.1 | |
| Strain, strain background (Mus musculus, male and female) | C57Bl/6 | Jackson Laboratories | 000664 | RRID:IMSR_JAX:000664 |
| Cell line (Mus musculus) | Ocy454 | P. Divieti-Pajevic, Boston University | RRID:CVCL_UW31 | |
| Cell line (Rattus norvegicus) | UMR106 | ATCC | CRL-1661 | RRID:CVCL_3617 |
| Transfected construct (Mus musculus) | CaMKII T286A | Addgene | #29430 | |
| Transfected construct (Homo sapiens) | GFP-tagged sclerostin | Origene | #RG217648 | |
| Transfected construct (Mus musculus) | myc-tagged sclerostin | Origene | #MR222588 | |
| Transfected construct (Mus musculus) | KillerRed | Evrogen | FP966 | |
| Biological sample (Homo sapiens) | WT and GD iPSCs | PMID:23071332 | ||
| Antibody | Goat Polyclonal Anti-sclerostin | R and D Systems | AF1589 RRID:AB_2270997 |
(1:250–500) |
| Antibody | Mouse Monoclonal Anti-GAPDH | Millipore | MAB374 RRID:AB_2107445 |
(1:2500) |
| Antibody | Mouse Monoclonal Anti-βActin | Sigma | A1978 RRID:AB_476692 |
(1:5000) |
| Antibody | Mouse Monoclonal Anti-αTubulin | Sigma | T9026 RRID:AB_477593 |
(1:2000) |
| Antibody | Rabbit Polyclonal Anti-Col1a1 | Sigma | ABT257 RRID:AB_2890134 |
(1:1000) |
| Antibody | Rabbit Monoclonal Anti-pCaMKII | Cell Signalling Technology | 12716S RRID:AB_2713889 |
(1:1000) |
| Antibody | Rabbit Polyclonal Anti-Total CaMKII | Cell Signalling Technology | 3362S RRID:AB_2067938 |
(1:1000) |
| Antibody | Rabbit Monoclonal Anti-p62/Sequestosome-1 | Cell Signalling Technology | 23214 RRID:AB_2798858 |
(1:250–500) |
| Antibody | Rabbit Polyclonal Anti-LC3B | Cell Signalling Technology | 2775 RRID:AB_915950 |
(1:500) |
| Antibody | Rabbit Monoclonal Anti-Rab27A | Cell Signalling Technology | 69295 RRID:AB_2799759 |
(1:250) |
| Antibody | HRP Anti-Rabbit | Cell Signalling Technology | 7074 RRID:AB_2099233 |
(1:1000–5000) |
| Antibody | HRP Anti-Mouse | Cell Signalling Technology | 7076 RRID:AB_330924 |
(1:1000–5000) |
| Antibody | HRP Anti-Goat | Thermo Fisher Scientific |
A27014 RRID:AB_2536079 |
(1:1000) |
| Antibody | Donkey anti-Goat IgG Alexa Fluor 546 | Thermo Fisher Scientific | A-11056 RRID:AB_142628 |
(1:100) |
| Antibody | Chicken anti-Rabbit Alexa Fluor 488 | Thermo Fisher Scientific | A-21441 RRID:AB_2535859 |
(1:100) |
| Sequence-based reagent | Sost | NCBI BLAST | NM_024449.6 | GGA ATG ATG CCA CAG AGG TCA T and CCC GGT TCA TGG TCT GGT T |
| Sequence-based reagent | Gapdh | NCBI BLAST | NG_007785.2 | CGT GTT CCT ACC CCC AAT GT and TGT CAT CAT ACT TGG CAG GTT TCT |
| Sequence-based reagent | Hprt | NCBI BLAST | NM_013556.2 | AGC AGT ACA GCC CCA AAA TGG and AAC AAA GTC TGG CCT GTA TCC AA |
| Sequence-based reagent | Rpl13 | NCBI BLAST | NM_016738.5 | CGA AAC AAG TCC ACG GAG TCA and GAG CTT GGA GCG GTA CTC CTT |
| Commercial assay or kit | Magic Red | Sigma | CS0370 | |
| Commercial assay or kit | siR-Lysosome | Spirochrome | SC016 | |
| Commercial assay or kit | Lysotracker | Thermo Fisher Scientific | L7528 | |
| Commercial assay or kit | High-Capacity RNA-to-cDNA Kit | Thermo Fisher Scientific | 4388950 | |
| Commercial assay or kit | Maxima SYBR Green/ROX qPCR Master Mix | Thermo Fisher Scientific | FERK0221 | |
| Commercial assay or kit | JetPrime Transfection kit | PolyPlus Transfection | 114–75 | |
| Chemical compound, drug | Bafilomycin A1 (in vitro studies) | Cell Signalling Technology | 54645 | |
| Chemical compound, drug | Bafilomycin A1 (in vivo studies) | Research Products International | 88899-55-2 | |
| Chemical compound, drug | Brefeldin A | Cell Signalling Technology | 9972 | |
| Chemical compound, drug | MG-132 | Cell Signalling Technology | 2194 | |
| Chemical compound, drug | Cycloheximide | Cell Signalling Technology | 2112 | |
| Chemical compound, drug | PTH (1–34) | US Biological Life Sciences | #P3109-24D | |
| Chemical compound, drug | Leupeptin | Millipore | EI8 | |
| Chemical compound, drug | Apocynin | Sigma | 178385 | |
| Chemical compound, drug | L-NAME | Millipore | N5751 | |
| Chemical compound, drug | SNAP | Sigma | N3398 | |
| Chemical compound, drug | Alizarin Red | Sigma | A3882 | |
| Chemical compound, drug | KN-93 | Sigma | K1358 | |
| Chemical compound, drug | Calcein | Sigma | C0875 | |
| Chemical compound, drug | Halt Protease and Phosphatase Inhibitor Cocktail (EDTA-free) | Thermo Fisher Scientific | 78440 | |
| Chemical compound, drug | SuperBlockPBS | Thermo Fisher Scientific | 37515 | |
| Software, algorithm | FIJI | ImageJ | RRID:SCR_002285 | |
| Software, algorithm | Nikon NIS Elements 5.2 | Nikon | RRID:SCR_014329 | |
| Other | REVERT Total Protein Stain | Licor | 827–15733 | |
| Other | Prolong Gold Antifade Reagent with DAPI | Cell Signalling Technology | 8961S | |
| Other | TRIzol | Sigma | T9424 | |
| Other | DCF | Invitrogen | D399 |