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. 2021 Mar 26;10:e60546. doi: 10.7554/eLife.60546

Key resources table.

Reagent type (species)
or resource
Designation Source or reference Identifiers Additional information
Strain, strain background (M. musculus) C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664
Strain, strain background (M. musculus) CD1 The Jackson Laboratory RRID: IMSR_JAX: 003814
Strain, strain background (M. musculus) Omp-IRES-tTA The Jackson Laboratory RRID: IMSR_JAX: 017754
Strain, strain background (M. musculus) tetO- Kcnj2-IRES-tauLacZ The Jackson Laboratory RRID: IMSR_JAX: 009136 Also known as: tetO- Kir2.1-IRES-tauLacZ
Strain, strain background (M. musculus) Olfr160-IRES-tauGFP The Jackson Laboratory RRID: IMSR_JAX:006678 Also known as:
M72-IRES-tauGFP
Other Amyl acetate Sigma-Aldrich CAS# 628-63-7 Abbr: AA
Other Hexanal Sigma-Aldrich CAS# 66-25-1; Abbr: HXH
Other 2-Pentanone Sigma-Aldrich CAS# 107-87-9 Abbr: PTO
Other Valeraldehyde Sigma-Aldrich CAS# 110-62-3 Abbr: VAH
Other Butyl acetate Sigma-Aldrich CAS# 123-86-4 Abbr: BAE
Other 2-Heptanone Sigma-Aldrich CAS# 110-43-0 Abbr: HPO
Other Methyl butyrate Sigma-Aldrich CAS# 623-42-7 Abbr: MBE
Other Methyl propionate Sigma-Aldrich CAS# 554-12-1 Abbr: MPE
Other R(−)-Carvone Sigma-Aldrich CAS# 6485-40-1 Abbr: (−) Car
Other S(+)-Carvone Sigma-Aldrich CAS# 2244-16-8 Abbr: (+) Car
Other Methyl caproate Sigma-Aldrich CAS# 106-70-7 Abbr: MCE
Other Heptanal Sigma-Aldrich CAS# 591-78-6 Abbr: HPH
Other Methyl valerate Sigma-Aldrich CAS# 624-24-8 Abbr: MVE
Other 2-Phenylethylamine Sigma-Aldrich CAS# 64-04-0 Abbr: PEA
Other Eugenol Sigma-Aldrich CAS# 97-53-0 Abbr: EUG
Other 2-Methylbutyric acid Sigma-Aldrich CAS# 116-53-0 Abbr: 2-MBA
Other Isoamylamine Sigma-Aldrich CAS# 107-85-7 Abbr: IAMM
Other Trimethylamine Sigma-Aldrich CAS# 1184-78-7 Abbr: TMA
Other Female mouse urine Fresh collected Strain: c57bl/6 Abbr: FU
Other Male mouse urine Fresh collected Strain: c57bl/6 Abbr: MU
Other Coyote urine Harmon scents CCHCY4 Abbr: CU
Other Peanut butter Jif Extra Crunchy Abbr: PB
Other Maple flavor Frontier natural products co-op #23081 Abbr: Maple
Other Lemon flavor Frontier natural products co-op #23071 Abbr: Lemon
Antibodies Donkey anti-Rabbit 488 Thermo Fisher Scientific R37118; RRID:AB_2556546 (1:1000)
Antibodies Rabbit anti-NCAM Millipore Sigma ABN2181-100UG (1:400)
Antibodies Goat anti_OMP Wako 544–10001; RRID:AB_2315007 (1:400)
Antibodies Rat anti-NCAM Millipore AB5032 (1:400)
Antibodies Chicken anti-GFP Abcam ab13970-100; RRID:AB_300798 (1:1000)
Antibodies Rabbit anti MOR28 Gilad Barnea Lab RRID:AB_2636804 (1:500)
Antibodies Guinea pig anti-TAAR4 Gilad Barnea Lab N/A (1:500)
Antibodies Rabbit anti-Phospho-S6 (Ser235/236) Cell Signaling 4854; RRID:AB_390782 (1:1000)
Antibodies Goat anti-Guinea Pig Alexa Fluor 488 Thermo Fisher Scientific A-11073; RRID:AB_2534117 (1:1000)
Antibodies Goat anti-Guinea Pig Alexa Fluor 568 Thermo Fisher Scientific A-11075; RRID:AB_2534119 (1:1000)
Antibodies DAPI Thermo Fisher Scientific D1306; RRID:AB_2629482 (1:1000)
Antibodies TOTO-3 Thermo Fisher Scientific T3604 (1:1000)
Antibodies Dextran, Biotin, 3000 MW, Lysine Fixable Thermo Fisher Scientific D7135
Antibodies Streptavidin, Alexa Fluor 568 conjugate Thermo Fisher Scientific S11226; RRID:AB_2315774 (1:1000)
Other Doxycycline diet Envigo TD.120046
Other C and B Metabond Quick Adhesive Cement System Parkell Cat# UN1247
Other 3 p peptide United Peptide biotin-QIAKRRRLpSpSLRApSTSKSESSQK 25 nM
Other Isoflurane Patterson Veterinary 07-893-1389;
CAS# 26675-46-7;
2–5%
Other Xylazine Patterson Veterinary 07-893-2121 10 mg/kg
Other Ketamine Vedco VINV-KETA-0VED 100 mg/kg
Software and algorithms OriginPro Origin Lab RRID:SCR_014212 https://www.originlab.com/Origin
Software and algorithms MATLAB Mathworks RRID:SCR_001622 https://www.mathworks.com/
Software and algorithms Custom MATLAB scripts Yu Lab N/A
Software and algorithms ImageJ (Fiji) software NIH RRID:SCR_002285
Software and algorithms R 4.0.3 R Project for Statistical Computing RRID:SCR_001905 https://www.r-project.org/
Software and algorithms Salmon https://combine-lab.github.io/salmon/
Software and algorithms ENSEMBL https://www.ensembl.org/index.html RRID:SCR_002344
Software and algorithms tximport https://www.bioconductor.org/packages/release/bioc/html/tximport.html RRID:SCR_016752
Software and algorithms DESeq2 https://bioconductor.org/packages/release/bioc/html/DESeq2.html RRID:SCR_015687
Software and algorithms GOSeq https://bioconductor.org/packages/release/bioc/html/goseq.html RRID:SCR_017052
Software and algorithms biomaRt https://bioconductor.org/packages/release/bioc/html/biomaRt.html RRID:SCR_019214
Software and algorithms Gephi https://gephi.org/ SCR_004293
Software and algorithms pheatmap https://rdrr.io/cran/pheatmap/ RRID:SCR_016418
Software and algorithms rgexf https://gvegayon.github.io/rgexf