Fig. 2.

Heat-inactivation and freeze-drying do not affect protein stability. (A) Control untreated viable cells. (B) Complete loss of viability on heat-inactivation. (C) Western blot image showing the effect of heat-inactivation on protein stability. Well, 1, 2 (untreated cycling cells); well 3 4 (heat-inactivated cells). (D) Proper loading and protein transfer is shown by Ponceau S-stain blot image. Western blot image showing the effect of freeze-drying on protein stability (E) well 1, 2 (control cycling cells); well 3,4 (lyophilized yeast cells). (F) Proper loading and protein transfer is shown by Ponceau S-stain blot image. Bottom free GFP bands are due to vacuolar protease (G) well 1 (PPY12 strain); well 2-3 (SMD1163, protease deficient strain, lack Prb1, cytosolic and Pep4, vacuolar protease). (H) Proper loading and protein transfer is shown by Ponceau S-stain blot image. Bands of interest are pointed by arrows towards them, while free GFP is pointed by an asterisk mark