Fig. 5.

Freeze and thaws do not affect the stability of the expressed protein. (A) Effect of freeze and thaw at −20 °C. Control (lyophilized powder stored at 30 °C), well 1,2 (one cycle of freeze and thaw), well 3,4 (two-cycle of freeze and thaw). (B) Effect of freeze and thaw at -80 °C. Same common control as above, well 1,2 (one cycle of freeze and thaw), well 3,4 (two-cycle of freeze and thaw). Note, in all cases, the protein amount was normalized based on the weight of powder and, each sample was taken in duplex. Loading and proper transfer are shown through Ponceau S- stained of same blots. Also, a control sample is common in panel A and B