Figure 2.

LIR reduces oxidative stress in random flaps. (a) Immunohistochemistry (IHC) was used to assess superoxygen dismutase 1 (SOD1) expression in each group (magnification: 200x; scale bar: 50 μm). (b) Histogram of SOD1 absorbance. (c–e) The expression of SOD1, haem oxidase 1 (HO1), and endothelial nitric oxide synthase (eNOS) was determined by Western blotting. (f–h) ImageJ was used to quantitatively analyse the optical density of SOD1, HO1, and eNOS in each group. The data are presented as the means ± standard error, n = 6 for each group. ^∗p < 0.05 and ^∗∗p < 0.01.