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. 2021 Mar 31;2021:6610603. doi: 10.1155/2021/6610603

Figure 3.

Figure 3

LIR inhibits pyroptosis in flap tissue. (a) Immunofluorescence (IF) assays were performed to evaluate caspase-1 levels in flaps from the control and LIR groups (scale bar: 10 μm). (b) Histogram of the optical density value of caspase-1. (c) Immunofluorescence detection at the gasdermin D-N-terminal domain (GSDMD-N) level in the flap (scale bar: 10 μm). (d) Histogram of GSDMD-N optical density values. (e) Western blot analysis of ASC, interleukin-18 (IL-18), interleukin-1β (IL-1β), caspase-1, GSDMD-N, and nucleotide binding oligomerization segment-like receptor family 3 (NLRP3) expression. (f) ImageJ was used to quantitatively analyse the optical density values of ASC, caspase-1, GSDMD-N, IL-1β, IL-18, and NLRP3 in each group. The data are presented as the means ± standard error, n = 6 for each group. ∗p < 0.05 and ∗∗p < 0.01.