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. 2021 Apr 1;23(4):413–423. doi: 10.1038/s41556-021-00637-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — a, Immunoblot analysis of PHD1/2/3-depleted HUVECs showing regulation of HIF targets and proliferation-associated proteins. Cells were generated by lentiviral transduction with FLAG-tagged Cas9, control- (gCtrl) or PHD1/2/3-targeting gRNAs (gPHD1/2/3). b, EdU-incorporation in PHD1/2/3-depleted HUVECs. DAPI was used to identify ECs nuclei. c, Quantification of EdU-incorporation in gCtrl and gPHD1/2/3-transduced HUVECs. Values represent the percentage of EdU-labelled ECs, (n=20, 24 independent samples for gCtrl and gPHD1/2/3). d, Reduced DNA synthesis in PHD1/2/3-depleted ECs, as assessed by analysing ³H-thymidine incorporation. Values are represented as fold-change relative to control, (n=6 independent samples). Western blot data in a were from the respective experiment, processed in parallel, and are representative of at least three independent experiments. c and d represent mean ± s.e.m.; a two-tailed unpaired t-test was used; **P< 0.01; ****P<0.0001. The numerical data, unprocessed western blots and P values are provided as source data.

Source data