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(A) Z’ factor measurements for eIF4E using three different probes (1a, 1b, and 1e). Assay conditions: 10 nM 1a, 100 nM eIF4E, 1 µM m7GTP; 1 nM 1b, 25 nM eIF4E, 1.5 µM m7GTP; 1 nM 1e, 100 nM eIF4E, 1.5 µM m7GTP. (B) Screening experiment for eIF4E (1 nM 1e, 100 nM eIF4E, 750 nM inhibitor).
