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. 2021 Apr 8;11:7777. doi: 10.1038/s41598-021-87462-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular inflammatory infiltrates into the parenchymal tissue. The upper panels are representative lung sections derived from mice treated with either saline (top) or LPS (bottom) through either the intranasal (IN) or the intratracheal (IT) route. The bar graph below depicts the mean inflammatory score in each group. Scale bars are 100 µm. The results of the two-way ANOVA are shown at the bottom of the graph. *Indicates statistically significant from the IN-exposed group treated with saline. †Indicates statistically significant from the group treated with saline exposed through the same route. n = 18 per group and the data shown are means ± SD.