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. 2021 Apr 8;11:7777. doi: 10.1038/s41598-021-87462-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Contractility of excised tracheas. The maximal force generated by excised tracheas in response to (A) a cumulative concentration of methacholine (MCh) ending with 10^–4 M or (B) a cumulative concentration of potassium chloride (KCl) ending with 160 mM are depicted for mice treated in vivo with either saline (black) or LPS (grey) through either the intranasal (IN) or the intratracheal (IT) route. The results of the two-way ANOVA are shown at the bottom of each graph. n = 12 per group and the data shown are means ± SD.