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. 2021 Apr 8;7:32. doi: 10.1038/s41522-021-00206-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a, b Gene clusters for flagellar assembly. V. vulnificus MO6-24/O genomes include four flagellin genes¹², flaC (VVM06_02255) and flaD (VVM06_02252) at region I and flaB (VVM06_00808) and flaA (VVM06_00809) at region II. c–f Identification of transcription initiation sites (TIS) and promoters. Primer-extension experiments using a primer specific to each fla gene (Supplementary Table 2) revealed TIS and the promoters of flaA (c), flaB (d), flaC (e), and flaD (f). Lane C, T, A, and G represent the nucleotide sequencing ladders of the upstream region for each gene. An arrow denotes the cDNA band indicating TIS. TIS (designated with +1) and its promoter (underlined and aligned with the consensus sequences for RpoN or RpoF^68,69) were indicated in nucleotide sequences upstream of the initiation codon (ATG) of each gene.