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. 2021 Apr 8;11:7702. doi: 10.1038/s41598-021-87295-8

Figure 1.

Figure 1

RT-QuIC reactions seeded with RLN homogenates from deer with RLNs that gave either negative, relatively weak, or strong ELISA readings as described in the main text. Replicate aliquots (n = 4) of the designated dilution of each homogenate were independently assayed in microplate wells with the thioflavin T fluorescence indicated in arbitrary relative units (a.u.) as a function of reaction time. Reactions seeded with 10–4 RLN tissue dilutions (w/v) from negative control deer (n = 5) or the designated dilutions from weak ELISA-positive (n = 5) and strong ELISA-positive deer (n = 5).