Table 2.

Primers used for cloning and generation of shuttle vectors using Gibson assembly cloning.

Constructa	Directionb	Sequence (5′ → 3′)
pc301B gC	Vector For	ACATATTACTTTCGTCCGTCGGTAAGCCTATCCCTAACCCTCTCC
	Vector Rev	GACGCGTGCATGGGGAAAATTCCGAGCTCGGTACCAAGCTTAACTAG
	Insert For	AGCTTGGTACCGAGCTCGGAATTTTCCCCATGCACGCGTCACG
	Insert Rev	GGGTTAGGGATAGGCTTACCGACGGACGAAAGTAATATGTATTTTTTCCCGG
pc3 × Flag301B gC	Vector For	ACAAGGATGACGACGATAAGATTAACCCCGATCTAGCTACACCC
	Vector Rev	CCGTCATGATCCTTGTAATCGCTAGCGCTTAGGACGCG
	Insert For	GCCGCGTCCTAAGCGCTAGCGATTACAAGGATCATGACGGAGATTACAAGG
	Insert Rev	GTAGCTAGATCGGGGTTAATCTTATCGTCGTCATCCTTGTAATCGATGT
pEP-301BgC-in	Vector For	GGCATAGAAATATCATCAGCCGAATATTACTCC
	Vector Rev	CGATTACCCTGTTATCCCTAGCTGATGATATTTCTATGCCGCTTGAG
	Insert 1 For	GGCATAGAAATATCATCAGCTAGGGATAACAGGGTAATCGATTTATTCAACAAAG
	Insert 1 Rev	CCTGCAAAGACCTGTAACCAGCCAGTGTTACAACCAATTAACCAAT
	Insert 2 For	TAATTGGTTGTAACACTGGCTGGTTACAGGTCTTTGCAGGACCC
	Insert 2 Rev	TCATCTTGGAGTAATATTCGGCTGATGATATT

^aConstruct generated with the set of primers.

^bDirectionality of the primer and product produced for Gibson assembly cloning.