. 2021 Apr 8;11:7753. doi: 10.1038/s41598-021-87400-x

Table 3.

Primers used for generation of recombinant Gallid alphaherpesvirus 3 strain 301B/1.

Modification^a	Direction^b	Sequence (5′- 3′)^c
UL47mRFP	Forward	AGAAGATGCGAAGGAGGCGATCTTCAAAAAAACGGACCGGATGGCCTCCTCCGAGGACG
UL47mRFP	Reverse	*TCACCACGATCTGCACGCCGCTCCGTGCGCTTTTTTTTTA*CAAGGCGCCGGTGGAGTG
ΔgC	Forward	ATATACGCTCTCGGAGACGCGGCTCGCACGCCAGCTGAAATATTTTCCCCTAGTTTGCGGTGACATTGATTAGGGATAACAGGGTAATCGATTT
ΔgC	Reverse	*TACAAGAGCTCGGGGCATATAATGAGCCAG*ATCAATGTCACCGCAAACTAGGGGAAAATATTTCAGCTGGGCCAGTGTTACAACCAATTAACC
ΔgC-R (3 × Flag301BgC)	Forward	GGCTCGCACGCCAGCTGAAATATTTTCCCCCCCATGCACGCGTCACG
ΔgC-R (3 × Flag301BgC)	Reverse	*AATGAGCCAGATCAATGTCACCGCAAA*CTAGACGGACGAAAGTAATATGTATTTTTTCCCG
MDV gC	Forward	ATATACGCTCTCGGAGACGCGGCTCGCACGTATCTTCCCTCATGCTCACG
MDV gC	Reverse	*TACAAGAGCTCGGGGCATATAATGAGCCAG*CATAACAATGAGATTATAAT

^aModification to the 301B/1 genome using two-step Red-mediated recombination.

^bDirectionality of the primer.

^cUnderlined sequence indicates start and stop codons for 301B/1 UL44 gene. Italics indicate the template-binding region of the primers for PCR amplification with pEP-mRFP-in, pEP-KanS2, pEP-301BgC-in, or pEP-MDVgC-in. Bold indicates unique upstream integration sequences. Bold italic indicates unique downstream integration sequences.