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. 2021 Apr 8;11:7700. doi: 10.1038/s41598-021-87307-7

Figure 3.

Figure 3

Dose-dependent GABAA-α5 receptor occupancy by basmisanil in rat brain. Dose-dependent blockade of [3H]-Ro 15-4513 binding by basmisanil determined 60 min after p.o. administration of basmisanil and 15 min after i.v. injection of [3H]-Ro 15-4513. (a) Sagittal brain sections of female Sprague–Dawley rats pretreated with vehicle (n = 4), L-655,708 (10 mg/kg; n = 4) or increasing doses of basmisanil at 3 mg/kg (n = 4), 10 mg/kg (n = 4), 30 mg/kg (n = 4) and 100 mg/kg (n = 2). (b) Quantitative autoradiography signals in the hippocampus were plotted against individual plasma concentrations of basmisanil. The curve was fitted to the experimental results using the Hill equation (Eq. (1) with Hill coefficient D = 1).