Table 1.

GSB-106 attenuates the apoptosis of serum withdrawn SH-SY5Y cells.

Groups	Apoptosis, % of cells		Necrosis
	Early	Late
10% FBS	0.5 ± 0.01	1.8 ± 0.1	2.75 ± 0.5
0% FBS	4.32 ± 0.2	7.08 ± 0.09	2.92 ± 0.4
BDNF, ~ 1 nmol	3.85 ± 0.1*	5.9 ± 0.1*	2.74 ± 0.5
GSB-106
10 nmol	3.68 ± 0.1*	5.85 ± 0.3*	3.15 ± 0.2
100 nmol	3.65 ± 0.09*	6.2 ± 0.3*	2.42 ± 0.6
1 µmol	3.9 ± 0.3	5.1 ± 0.2*	2.82 ± 0.4
10 µmol	5.2 ± 0.2	8.91 ± 0.1	2.81 ± 0.5
100 µmol	5.67 ± 0.1	8.18 ± 0.3	2.44 ± 0.3

SH-SY5Y cells were cultured in serum-free culture medium (0% FBS) and treated with BDNF.

(~ 1 nmol) or GSB-106 (10 nmol to 100 µmol) for 72 h. After incubation cells were collected and stained with Metabolic Activity Dead Cell Apoptosis Kit with C12 Resazurin, Annexin V APC, and SYTOX Green according to the manufacturer's recommendations. The stages of apoptosis were determined by staining: early—Annexin V⁺/SYTO Green⁻; late—Annexin V⁺/SYTO Green⁺; necrosis—Annexin V⁻/SYTO Green⁺. The data is presented as the mean ± S.E.M. of percentage of total cell population (n = 3; *p < 0.05 compared with the “0% FBS” group; Wilcoxon t-test).