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. 2021 Apr 8;12:2111. doi: 10.1038/s41467-021-22217-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic representation of replication intermediates arising at termination regions as observed by 2D gel electrophoresis. The migration pattern of different replication intermediates referred to in the text is illustrated. b Schematic representation of the NPS302/TER302 region analysed by 2D gel electrophoresis. c Visualization of replication intermediates by 2D gel electrophoresis from cells of the indicated genotype synchronously released from G1 in media containing 200 mM HU, as well as Auxin (Aux) and Tetracycline (Tc). FACS profiles indicated on the right show cell cycle progression. Sgs1 and Top3 tagged with HA were depleted with Auxin and Tetracycline as indicated by Western blots. Pgk1 was used as loading control. Joint molecules accumulating on the X-arc are indicated by red arrows. The experiment was performed reproducibly twice. See also Supplementary Figs. 3 and 4. Source data are provided as a Source Data file.