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. 2021 Mar 26;15:649982. doi: 10.3389/fnins.2021.649982

FIGURE 1.

FIGURE 1

PIT-stroke induced decreased levels of miR-124 and increased DAPK1 levels. (A) Representative graph of 2,3,5-triphenyltetrazolium chloride (TTC) staining showing cerebral infarct in PIT-stroke. (B) Changes in miR-124 expression levels at 0.5, 2, 4, 6, 12, 24, 48, and 72 h after PIT-stroke (n = 3/group). (C) TdT-mediated dUTP nick end labeling (TUNEL) staining (red) in primary cultured neurons [14 days in vitro (DIV)] was performed after an oxygen glucose deprivation (OGD) challenge (scale bar = 50 μm) and quantified (D) (n = 4/group). (E) Bar graph shows the relative expression of miR-124 following OGD. (F) Predicted binding sites of miR-124 in DAPK1 3′-UTR and its mutant. (G) Human embryonic kidney 293 (HEK293) cells were co-transfected with miR-124 or control miRNA with wild-type (WT) or mutated (scrambled) 3′-UTR of DAPK1. Data are presented as mean ± SD (n = 6/group). *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham or Con or scramble group.