Figure 2.

SQV decreases the intracellular survival of Mycobacterium tuberculosis (Mtb). (A) Intracellular survival of Mtb during mono- or co-infection with HIV along 7 days of infection. Data represents colony forming units (CFU) of intracellular bacteria recovered from Mø treated with the PI or DMSO control. Culture medium was changed on day 3 p.i. without addition of fresh PIs. Values depict mean CFU representative of three biological replicates from one representative experiment performed in triplicate. Error bars depict the standard deviation (P** < 0.01; ***P < 0.001 relatively to control). (B) Percentage of Mø infected with Mtb and median fluorescence intensity of Mtb per Mø were measured by flow cytometry in Mø pre-treated with the PIs and after 3 h of infection with GFP-expressing Mtb. Bar-plots depict the average of three biological replicates and the error bars depict the standard error. Raw values from one representative replicate are presented in the fluorescence intensity histograms. (C) Mtb growth curves in broth medium treated with PIs and incubated for 15 days. Values represent the optical density measured at discrete time points from one representative experiment performed twice. Isoniazid (INH) was used as control for inhibition of growth.