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. 2021 Mar 26;13:612856. doi: 10.3389/fnagi.2021.612856

Figure 4.

Figure 4

Increased expression of regenerative genes and brain protection after ND1 treatment. Western blotting measured several key growth factors and synaptic proteins in the peri-infarcted region following stoke in control and ND1-treated animals 6 weeks after stroke. (A,B) The expression of BDNF (unpaired two-tailed Student’s t-test: n = 8/group, t = 2.305, *p < 0.05) and FGF (unpaired two-tailed Student’s t-test: n = 8/group, t = 2.407, *p < 0.05) were significantly increased in the brain subjected to ND1 vs. vehicle control of stroke mice. Panels (A,B) were performed in the same Western blot experiments with the same load control. (C) The expression of postsynaptic protein PSD-95 decreased after stroke and ND1 treatment partially recovered this synaptic protein loss. Two-way ANOVA (interaction: F(2,4) = 45.52, ***p = 0.0018 followed by Holm–Sidak’s Multiple Comparisons Test: *p < 0.05 vs. sham; #p < 0.05 vs. empty vector control). (D) The ND1 treatment significantly reduced the NF-kB level in the post-stroke brain (unpaired two-tailed Student’s t-test: n = 3 for vector control and n = 6 for ND1 group, t = 3.613, **p < 0.01).