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. 2021 Mar 26;8:650881. doi: 10.3389/fmolb.2021.650881

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Copyright © 2021 Thapa, Roivas, Haataja, Permi and Pentikäinen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chemical shift perturbation of phosphomimetic mutants of ARPP-16 and ARPP19 (A) and (B) Chemical shift perturbation due to S46E and S88E mutations, respectively. (C,D) Chemical shift perturbation due to S62E and S104E mutations, respectively. Δδ [ppm] refers to the combined HN and N chemical shift changes, according to the equation: Δδ(HN, N) = sqrt[(ΔδHN)² + 0.2(ΔδN)²]. Red arrow indicates the phosphomimetic mutation site. Positions of α-helices and the A-subunit (PR65) binding site are shown.