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Human SS plasma induces IL-8 secretion in HUVEC through TLR4 signaling. (A) HUVEC were cultured with 2% AA (n=9) or SS (n=13) plasma in RPMI-1640 media for 18 hours. The IL-8 content in the conditioned medium was measured by ELISA. (B) HUVEC were cultured with 2% SS plasma plus vehicle or TLR4 signaling inhibitor, TAK-242 (1 µM) for 18 hours and IL-8 secretion into the media was measured by ELISA (n=6). Bars are means ± SD, *p<0.002, #p<0.005.
