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. 2021 Mar 26;12:632709. doi: 10.3389/fimmu.2021.632709

Figure 5.

Figure 5

Hemopexin inhibits IL-8 secretion by HUVEC stimulated with human SS-plasma and AA-plasma plus sMD-2. (A) HUVEC media containing human AA or SS plasma (2%) was pre-incubated with recombinant hemopexin (HPX, 10 µM) for 30 minutes before being added to HUVEC for 18 hours. The IL-8 secreted into the HUVEC media was measured by ELISA (n=4). (B) CHO cells expressing recombinant MD-2 were cultured in protein-free medium for 2 days, the medium was collected as sMD-2-conditioned medium. HUVEC media containing AA (n=7) or SS (n=5) plasma (2%) plus sMD-2-conditioned medium (10%) was pre-incubated with HPX (10 µM) for 30 minutes before being added to HUVEC for 18 hours. The secreted IL-8 was measured by ELISA and presented as fold-change compared to AA-plasma +control conditioned medium without HPX. Bars are means ± SD. *p<0.05 and **p<0.01.