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. 2021 Jan 15;30(1):72–77. doi: 10.1093/hmg/ddab014

Figure 2.

Figure 2

Effect of temperature and imatinib treatment on PDGFRB and downstream signalling. Panel A: levels of phosphorylated PDGFRB measured by ELISA analysis. In p.(Asn666Tyr) and p.(Asn666Ser) fibroblasts, increased levels of phosphorylated PDGFRB were present at 37°C. Only p.(Asn666Tyr) fibroblasts were temperature sensitive, with increasing levels after incubation at 32°C overnight. Panel B: increased levels of downstream ligands, P-AKTSer473, P-PLCγ1 and P-STAT1, at 37°C in p.(Asn666Tyr) fibroblasts. Incubation at 32°C led to higher levels of P-AKT (P-AKTSer473 and P-AKTThr308) and P-PLCγ1, but not P-STAT1. Imatinib treatment of p.(Asn666Tyr) fibroblasts reduced levels of P-AKT (P-AKTSer473 and P-AKTThr308) and P-PLCγ1, but not P-STAT1. In p.(Asn666Ser) fibroblasts increased levels of P-AKTSer473, P-PLCγ1 and P-STAT1 and STAT1 were present at 37°C, but only P-STAT1 had further greatly increased levels at 32°C. There was no difference on P-MAPK3 signalling.