Figure 3.
BBSome subcomplex consisting of BBS1, BBS5, BBS8 and BBS9 is recruited to photoreceptor cilia in the absence of BBS2 in Bbs2−/− retinal photoreceptors. (a) Sucrose gradient fractionation shows that the peak fractions of BBS1, BBS5, BBS8 and BBS9 are shifted from fraction 13 to fraction 11 in retinas of Bbs2−/− mice. (b) BBS8, a member of this subcomplex, localizes to photoreceptor ciliary axoneme in Bbs2−/− mice (arrow). (c) BBS9, a member of this subcomplex, is also observed in photoreceptor ciliary axoneme in Bbs2−/− mice (arrow).