Figure 5.
UC-MSCs enhance the function of MDSCs via arginase-1. MDSCs isolated from the spleens of control mice, septic mice, MSCs-treated septic mice, imipenem-treated septic mice, and MSCs + imipenem-treated septic mice were cocultured with equal numbers of LPS (10 ng/mL)-stimulated BMDMs (5×105 cells/well) for 24 h. (A,B,C,D) The levels of TNF-α, IL-6, IL-1β, and IL-10 in the supernatant were determined by ELISA. The data are presented as the mean ± SEM (n=5). The data shown in this figure are representative of three independent experiments. *, P<0.05; **, P<0.01; ***, P<0.001. UC-MSC, umbilical cord mesenchymal stem cell; MDSC, myeloid-derived suppressor cell; LPS, lipopolysaccharide; BMDM, bone marrow-derived macrophage; TNF-α, tumor necrosis factor-α; IL, interleukin; ELISA, enzyme-linked immunosorbent assay.