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. 2021 Mar;9(5):394. doi: 10.21037/atm-20-6456

Figure 6.

Figure 6

HemECs internalized HemSCs-exos transfected with miR-196b-5p mimic or inhibitor. (A) qRT-PCR confirmed HemSCs-exos were successfully transfected with miR-196b-5p mimic or inhibitor. (B) CCK-8 assay reflected the viability of HemECs. (C,D) Flow cytometry was used to determine cell apoptosis rate and the cells arrested in the G0/G1 phase. (E) Tube formation assay was conducted to examine the angiogenesis ability of HemECs. a, b, c, d represented miR-196b-5p mimic, NC mimic, NC inhibitor and miR-196b-5p inhibitor group, respectively. Scale bar = 100 µm. (F,G) qRT-PCR showed the expression pattern of miR-196b-5p and CDKN1B. Western blot presented that the expression levels of cyclin E, bcl-2, p27, Bax. All experiments were repeated at least 3 times independently. Results are shown as mean ± standard deviation. *P<0.05.