Fig. 6.

CD8 T cells were decreased in the HFD DIO E0771 model of triple-negative breast cancer, and tumor growth advantage was lost when the T-cell compartment was lost in the TKO model. (A,B) Box and whisker plots with all data points shown (mean, minimum to maximum). Unpaired Student's t-tests were not adjusted, with s.d. assumed between chow and HFD for individual cell subsets. E0771 (n=9/8), Wnt1 (n=6/6), TeLi (n=5/5), C11 (n=8/3), UN-KC (n=5/2). (A) CD4/CD8 ratio for each tumor model. (B) Percentage of CD8 T cells of total T cells. (C) CITRUS SAM results for E0771 model. CITRUS clusters that are significantly different between chow and HFD are not blue and are circled with a gray background (n=9/8). (D) Selected significant CITRUS clusters were plotted back onto the viSNE map. Plotted clusters are color coded to match the CITRUS plot in C. viSNE data shown are concatenated data for the ten CITRUS clusters and total cell numbers for the E0771 model. The black line indicates the divide between myeloid and lymphoid lineage cells/clusters. (E,F) Tumor growth volume over time for E0771 WT (n=4/4; E) and E0771 TKO (n=5/5; F) cohorts (mean±s.d.). Unpaired Student's t-tests were not adjusted, with s.d. assumed. (G) Final tumor masses for C11 tumors grown in TKO mice, showing that the HFD tumor growth difference remains (n=5/5). The appropriate comparison is to the C11 tumor masses for C11_1 and C11_2 batches shown in Fig. 1C. Data are graphed as scatter dot plots (mean±s.d.). Unpaired Student's t-test, with s.d. assumed.