KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-OT neurophysin | Whitnall et al., 1985 Ben-Barak et al., 1985; gift from Harold Gainer, PhD | PS38 |
| Alexa 488 donkey anti-mouse | Life Technologies | A-21202 |
| Alexa 647 donkey anti-mouse | Life Technologies | A-31571 |
| Alexa 647 goat anti-mouse | Life Technologies | A-21235 |
| Alexa 350 goat anti-mouse | Life Technologies | A-11045 |
| Rabbit anti-FluoroGold | Fluorochrome | NA |
| Alexa 647 donkey anti-rabbit | Life Technologies | A-31573 |
| Chicken anti-GFP | ABCAM | Ab13970 |
| Alexa 488 goat anti-chicken | Life Technologies | A-11039 |
| Goat anti-mCherry | SICGEN | AB0040 |
| Alexa 555 donkey anti-goat | Life Technologies | A-21432 |
| Mouse anti-FMRP | Developmental Studies Hybridoma Bank | 2F5-1 |
| Alexa 647 goat anti-mouse IgG2b | Jackson ImmunoResearch | 115-607-187; RRID: AB 2632546 |
| Rabbit anti-OT | Altstein and Gainer, 1988; gift from Harold Gainer, PhD | VA10 |
| Alexa 488 donkey anti-rabbit | Life Technologies | A-21206 |
| Bacterial and Virus Strains | ||
| rgAAV-EF1 a-fDIO-Cre | Schneeberger et al., 2019 | Addgene 121675- AAVrg |
| rgAAV-CAG-fDIO-GFP-Cre | Stanford University Neuroscience Gene Vector and Virus Core | N/A |
| CAV-2-GFP | Plateforme de Vectorologie de Montpellier | N/A |
| CAV-2-Cre-GFP | Plateforme de Vectorologie de Montpellier | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Neurobiotin | Vector Laboratories | SP-1120 |
| Alexa 350 streptavidin conjugate | Life Technologies | S11249 |
| FluoroGold | Fluorochrome | N/A |
| Red Retrobeads | Lumafluor | N/A |
| 4-Aminopyridine | Sigma-Aldrich | 275875 |
| Cocaine hydrochloride | Sigma-Aldrich | C5776 |
| Critical Commercial Assays | ||
| Worthington Papain Dissociation System | Worthington Biochemical Corporation | Cat#LK003182 |
| Illumina TruSeq kit | Illumina | Cat#RS-122-200 |
| Deposited Data | ||
| Accession number GSE147092 | GEO | |
| Experimental Models: Organisms/Strains | ||
| C57BL6/J | Jackson Laboratories | Stock # 000664 |
| Calbl-IRES-Cre | Jackson Laboratories | Stock # 028532 |
| Flp-dependent GFP reporter mice | Mutant Mouse Resource Center | Stock # 32038 |
| Ai9 Cre-dependent TdTomato reporter mice | Jackson Laboratories | Stock # 007909 |
| Constitutive Fmr1 knockout | Jackson Laboratories | Stock # 003025 |
| Conditional Fmr1 knockout | Mientjes et al., 2006 | N/A |
| Oxytocin-2A-Flp-optimized | Nardou et al., 2019 | N/A |
| Oligonucleotides | ||
| see supplemental tables (HCR) | ||
| Recombinant DNA | ||
| fDIO-Cre-GFP | Penzo et al., 2015 | Dr. Bo Li and Dr. Linda Van Aelst |
| Software and Algorithms | ||
| R version 3.5 | The R project | https://www.r-project.org/ |
| MATLAB | Mathworks | https://www.mathworks.com/ |
| Prism 5 | GraphPad | https://www.graphpad.com/ |
| Igor Pro | WaveMetrics | https://www.wavemetrics.com/ |
| Recording Artist (plugin for Igor Pro) | Richard C Gerkin, PhD | https://github.com/rgerkin/recording-artist |