p‐Histone H2A. XSer139 (20E3) Cell Signaling 9718 |
1:2,000 |
LC3A/B (D3U4C) Cell Signaling 12741 |
1:1,000 |
VCP Abcam ab11433 |
1:10,000 |
Acetyl Histone H3 Millipore 06‐599 |
1:1,000 |
GAPDH Sigma‐Aldrich G8795 |
1:10,000 |
TFE3 Cell Signaling 14779 |
1:1,000 |
MITF Cell Signaling 12590 |
1:1,000 |
CHOP Cell Signaling 2895 |
1:500 |
p‐eIF2α Cell Signaling 9721 |
1:500 |
eIF2α Cell Signaling 9722 |
1:1,000 |
ATF4 Cell Signaling 11815 |
1:1,000 |
UPP1 Abcam ab205031 |
1:1,000 |
Vinculin Abcam ab155120 |
1:1,000 |
LC3B Cell Signaling 2775 |
for immunofluorescence 1:200, for immunoblot 1:1,000 |
P62 BD Transduction Lab 610832 |
1:1,000 |
Lamp1 BD Pharmingen 555798 |
for immunofluorescence 1:100 |
Goat anti‐Rabbit IgG (H + L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific A21429 |
1:1,000 |
Donkey anti‐Mouse IgG (H + L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific A31570
|
1:1,000 |
Goat anti‐Mouse IgG (H + L) Cross‐Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific A21235 |
1:1,000 |
Alexa Fluor ™ 488 phalloidin Thermo Fisher Scientific A12379 |
1:800 |
DAPI (4′,6‐diamidino‐2‐phenylindole) Thermo Fisher Scientific D1306 |
1:10,000 |