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. 2021 Feb 22;13(4):e13392. doi: 10.15252/emmm.202013392

Figure EV5. Subretinal injection of AAV2.GL and AAV2.NN in mouse.

Figure EV5

Vector mediated eGFP expression and lateral spreading after subretinal injection in 1‐month‐old mouse retinas.
  1. Schematic depiction of subretinal injection in wild‐type mice followed by weekly in vivo cSLO examinations of the mouse retina from 1 to 4 weeks post‐injection (WPI) of 3E9 total vg (in 1 µl) of sc‐CMV‐eGFP packaged with AAV2.GL and AAV2.NN capsid variants.
  2. Schematic depiction of central locus shown in adjacent confocal scans of retinal cross sections immunolabelled for eGFP and DAPI, reporting transduction efficacy of AAV2.GL (left) and AAV2.NN (right).
  3. Schematic depiction of peripheral locus shown in adjacent immunolabelled confocal scans, as in (B).

Data information: Acquisition settings were kept constant for all samples. GFP = eGFP; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 50 μm.

Source data are available online for this figure.