| Plate |
|
|
|
| GEL-8100 |
Nusil Technology |
GEL-8100 |
High Purity Dielectric, Soft Silicone Gel kit |
| Dow Corning Sylgard 184 Silicone Encapsulant Clear 0.5 kg Kit |
Ellsworth Adhesives |
184 SIL ELAST KIT 0.5KG |
curing agent |
| Custom Cut Glass |
Hausser Scientific Company |
|
109.6mm± × 72.8mm± × 1mm thickness |
| Target 2TM Nylon Syringe Filter |
ThermoFisher Scientific |
F2513-4 |
|
| 96-well Stripwell Egg Crate Strip Holder |
Corning |
2572 |
|
| Polystyrene Universal Microplate Lid With Corner Notch |
Corning |
3099 |
|
| Ethyl alcohol |
Greenfield Global |
P016EA95 |
0.95 |
| 2-Propanol |
Sigma-Aldrich |
190764 |
ACS reagent, ≥99.5% |
| Surface Coating |
|
|
|
| Sulfo-SANPAH Crosslinker |
Proteochem |
c1111-100mg |
|
| Fibronectin bovine plasma |
Sigma-Aldrich |
F1141-1MG |
solution, sterile-filtered, BioReagent, suitable for cell culture |
| PBS, 1X |
Wisent |
319-005-CL |
pH 7.4, without calcium and magnesium |
| DMSO |
Sigma-Aldrich |
472301 |
|
| Cell Culture |
|
|
|
| DMEM, 1X |
Wisent |
319-005-CL |
4.5g/L glucose, with L-glutamine, sodium pyruvate and phenol red |
| FBS (Fetal Bovine Serum) |
Wisent |
080-150 |
Premium Quality, Endotoxin <1, Hemoglobin <25 |
| HEPES |
Wisent |
330-050-EL |
1M, free acid |
| Human Insulin Recombinant |
Wisent |
511-016-CM |
USP grade |
| Penicillin-Streptomycin Solution |
Wisent |
450-201-EL |
100 X, sterile filtered for cell culture |
| L-Glutamine solution |
Wisent |
609-065-EL |
200mM solution, sterile filtered for cell culture |
| Amphotericine B |
Wisent |
450-105-QL |
250μg/ml, sterile filtered for cell culture |
| Recombinant Human TGF-β1 |
Peprotech |
100-21 |
HEK293 Derived |
| Acetic acid |
Sigma-Aldrich |
537020 |
Glacial, ≥99.85% |
| Cictric acid |
Sigma-Aldrich |
251275 |
ACS reagent, ≥99.5% |
| NMuMG |
ATCC |
CRL-1636 |
Mouse Mammary Gland Cell Line |
| Sodium azide |
Fisher Schientific |
AC190385000 |
99%, extra pure, ACROS Organics |
| Potassium hydroxide |
Sigma-Aldrich |
221473 |
ACS reagent, ≥85%, pellets |
| TritonX-100 |
Sigma-Aldrich |
X100 |
laboratory grade |
| Bead Synthesis |
|
|
|
| 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (Dil) |
Sigma-Aldrich |
468495-100MG |
97% |
| Methyl methacrylate |
Sigma-Aldrich |
M55909-500ML |
contains ≤30 ppm MEHQ as inhibitor, 99% |
| Inhibitor Remover |
Sigma-Aldrich |
306312-1EA |
Prepacked column for removing hydroquinone and monomethyl ether hydroquinone |
| Methacryloxylpropyl Terminated Polydimethylsiloxane |
Gelest |
DMS-R31 (25,000g/mol) |
Polydimethylsiloxane stabilizer, 25,000g/mol, 1,000 cSt |
| 2,2′-Azobis(2-methylpropionitrile) (AIBN) |
Sigma-Aldrich |
441090-25G |
98% |
| Hexane |
Sigma-Aldrich |
296090-2L |
anhydrous, 95% |
| Hexane, mixture of isomers |
Sigma-Aldrich |
227064-1L |
anhydrous, ≥99% |
| Whatman qualitative filter paper, Grade 1 |
Sigma-Aldrich |
WHA1001055 |
circles, diam. 55 mm, |
| Equipment |
|
|
|
| Laurell WS-650Mz-23NPPB |
Laurell Technologies |
|
|
| UVP Handheld UV Lamp Model UVGL-58 |
VWR |
21474-622 |
|
| Rheometer |
Anton Paar |
MCR 302 WESP |
|
