Fig. 2.

FZD2 is critical for BC cell growth, migration, invasion and apoptosis in vitro. a FZD2 mRNA and protein were measured in BC cells with normal MCF-10A cell as control. b Knockdown of FZD2 induced by specific siRNAs in MDA-MB-231 and SK-BR-3 cells was identified by qRT-PCR and western blot. c CCK-8 assay was implemented to assess cell growth after inhibition of FZD2 expression. d The number of colonies was quantified after siRNA transfection in two BC cells. e Cell cycle distribution was evaluated by flow cytometry in two BC cells with FZD2 silencing. f Flow cytometry analysis of apoptosis in BC cells transfected with si-NC or FZD2-specific siRNAs. g Western blot was conducted to detect apoptosis-related proteins in BC cells with FZD2 silencing. h Representative images and quantitative bar graphs of wound-healing distance for FZD2-silenced cells. i Representative micrographs and quantification of the invaded or migrated cells after FZD2 knockdown. Results were obtained from Matrigel-coated transwell assays and non-Matrigel-coated transwell assays. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 were symbols of statistical significance. FZD2, frizzled class receptor 2; BC, breast cancer