Table 2.

Fusion genes by NGS and PCR and Chromosome karyotype analysis in FLT3 mutant AML

Fusion gene by NGS	Fusion gene by PCR	Chromosome karyotype	FLT3 mutation
AML1/ETO	AML1/ETO	46, XY, t(8;21)(q22;q22)[20]	FLT3-ITD, FLT3-TKD
AML1/ETO	AML1/ETO	46, X, -X,t(8;21)(q22;q22), del(9)(q22)[9]/46,XX,t(8;21)(q22;q22)[11]	FLT3-ITD
AML1/ETO	AML1/ETO	45, X, -X,t(8;21)[20]	FLT3-ITD
MLL-PTD	MLL-PTD	46, XY[20]	FLT3-ITD
MLL-PTD	MLL-PTD	46, XY[20]	FLT3-ITD
MLL-PTD	MLL-PTD	46, XX[20]	FLT3-ITD
MLL-PTD	MLL-PTD	47, XY, + 8?[10]/46, XY[10]	FLT3-ITD
MLL/AF9	MLL/AF9	46, XX,?der(2)(q11),inc[1] /46,XX[28]/hypodiploid [4] (44–45)	FLT3-ITD
MLL/AF9	MLL/AF9	47, XY, + 8[7]	FLT3-ITD
MLL/ELL	MLL/ELL	46, XX, t(11;19)(q23; q13)[10]	FLT3-ITD
NUP98/HOXA9	NUP98/HOXA9	NA	FLT3-ITD
NUP98/HOXA9	NUP98/HOXA9	46, XX[20]	FLT3-ITD
NUP98/HOXA9	NUP98/HOXA9	NA	FLT3-ITD
NUP98-NSD1	–	46, XX[20]	FLT3-ITD
NUP98-NSD1	–	47, XX, + 6[14]/46, XX[6]	FLT3-ITD
NUP98-NSD1	–	46, XY[20]	FLT3-ITD
NUP98-NSD1	–	46, XY[25]	FLT3-ITD
DEK/CAN	DEK/CAN	46, XX[20]	FLT3-ITD
DEK/CAN	DEK/CAN	46, XY, ?t(6;9)(p23;34)[10]/46, XY,?t(6;9)(p23;q34),?del(8)(q21)[11]/46,XY[1]	FLT3-ITD
PRDM16-SKI	–	46, XX[20]	FLT3-ITD
EFCAB2-ZNF238	–	46, XX [20]	FLT3-ITD
BCR/ABL	BCR/ABL	NA	FLT3-TKD
AML1/ETO	AML1/ETO	45, X, -Y, t(8;21)(q22;q22)[22]	FLT3-TKD
AML1/ETO	AML1/ETO	46, XY,t(8;21)(q22;q22)[26]/46,XY[1]	FLT3-TKD
AML1/ETO	AML1/ETO	46, XX, t(8;21)(q22;q22)[20]	FLT3-TKD
AML1/ETO	AML1/ETO	45,X,?Xq-,?8q-,-22[1]/43,X,?Xq-,-8,-10,-22[1]/45,X,-X[1]/47,XX, + mar[1]/40,-X,-X,-11,-21,-22, + mar[1]/46,XX[4]	FLT3-TKD
MLL/MLLT3	–	46, XX[20]	FLT3-TKD
DEK/CAN	DEK/CAN	47, XY, chtb(4)(?q31),? + 9,-15,inc[1]/46, XY[27]/hypodiploid [2] (44–45)	FLT3-TKD
AML/MDS1	–	46, XY [20]	FLT3-TKD
SLC45A3/ELK4	–	47, XY, + 8[7]	FLT3-TKD
MLL/TMX2-CTNND1	–	42–47,XY, + 3,del(3)(p13),del(3)(q13),-4,?add(4)(q35),-8,-11,dic(11;?)(q25;?),-16,-17,-18,-19,-20, + r, + mar1, + mar2, + mar3,inc[cp22]/46,XY[1]	FLT3-TKD
CBFB/MYH11	CBFB/MYH11	47, XY, + 22[2]/46, XY[23]	FLT3-TKD

NGS, next generation sequencing; PCR, polymerase chain reaction