Fig. 3.

Moracin O and P inhibit the TLR ligand-induced NF-κB activation in RAW264.7 cells. a Chemical structure of Moracin O and Moracin P. b RAW264.7-NFκB-Luc2 cells (5 × 10⁴ cells/well) were seeded onto 96-well plates and pretreated with Moracin O or Moracin P (0.3,3,10,100 or1000 nM). After 1 h, they were stimulated with each TLR ligand (IMQ: 10 μg/mL, polyI:C: 10 μg/mL or LPS: 10 ng/mL) for 6 h. The activity of NF-κB was measured using IVIS imaging system. The inhibitory effects on NF-κB activation were assessed relative to NF-κB activation in untreated control