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Primer sequences used in RT-PCR
| Gene | Primer sequence (5′–3′) |
|---|---|
| Bcl-2 | Forward: GACGACTTCTCCCGGCGCTA |
| Reverse: ACACATGACCCCACCGAAC | |
| Bax | Forward: CACCAAGAAGCTGAGCGAGT |
| Reverse: GCAAAGTAAAACAGGGCGACA | |
| Caspase-3 | Forward: AGATGTAAATGCAGCAAACCTC |
| Reverse: TCCTTCATCACCGTGGCTT | |
| Caspase-9 | Forward: ACATCCTCGTGTCCTACTCC |
| Reverse: TTGTAAATCCCTCGCTCGGAA | |
| GAPDH | Forward: GTTGTCGCCATCAATGATCCA |
| Reverse: TTCCCGTTCTCAGCCTTGACC |
