Figure 5.

CNS infiltrating CD45⁺ cells at the peak of disease in EAE female C57BL/6 mice treated with vehicle or WOBE437. Lymphocytes were gated considering their (A) CD45^high expression, then gated in (B) CD4⁺IFNγ⁺IL-17⁺ and (C) CD8⁺IFNγ⁺IL-17⁺ T cell subpopulations. Microglia cells were estimated by gating a (D) CD45^dim cell population. Infiltrating immune cells were isolated from brain or spinal cord collected at the peak of disease (4–6 days post-treatment, day 1 = onset). Daily treatment (i.p., 20 μL) with vehicle (DMSO) or WOBE437 (10 mg/kg) was started at the onset of disease. Data show mean ± SD of infiltrating immune cells isolated from the brain or spinal cord of n = 6 mice per group. Data is depicted as a summary of three independent experiments including n = 2 mice (pooled) per group. Isolated cells were resuspended in 1 mL of FACS buffer, and cellular count was assessed using the volumetric sample and sheath fluid delivery system of the Attune NxT cytometer. Statistical differences were determined using Mann–Whitney test. *, p < 0.05 compared to vehicle group.