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. 2021 Mar 26;4(2):765–779. doi: 10.1021/acsptsci.0c00214

Figure 7.

Figure 7

WOBE437 induced muscle relaxation without sedative effects in male C57BL/6 mice. (A) Muscle relaxation was evaluated using the Straub test after morphine administration (40 mg/kg, s.c.). (B) Spontaneous locomotion was evaluated in the open-field test (OFT) by placing each mouse in the arena and allowing it to freely move for 5 min. (C) The potential induction of catalepsy-like behavior was assessed in the bar test. Vehicle (DMSO, n = 10), WOBE437 (10 mg/kg, WOBE, n = 6), and WIN-55212-2 (3 mg/kg, WIN, n = 5) were injected (i.p.) 1 h before morphine injection. Baclofen (5 mg/kg, n = 7) and/or diazepam (6 mg/kg, DZP, n = 5) were injected (i.p.) 40 min before morphine injection. Importantly, the OFT and the bar test were conducted just before morphine administration: 8 and ∼2 min before, respectively. Data show (A) cumulative frequency or (B, C) median, percentile 25, percentile 75, minimum, and maximum. Statistical differences were determined using (A) the chi-square test and Fisher’s exact test or (B, C) the Kruskal–Wallis test followed by the Mann–Whitney test as post hoc analysis. *, p < 0.05 compared to vehicle group.