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. 2021 Apr 9;36(5):890–900. doi: 10.1007/s12250-021-00369-9

Fig. 1.

Fig. 1

Assembly of a SARS-CoV-2-GFP replicon. A Genome structure of SARS-CoV-2 and workflow of fragment generation to assemble a SARS-CoV-2-GFP replicon. The open reading frames (ORFs) of viral genes and sequences of untranslated regions (UTRs) are indicated. B Strategy for transformation-associated recombination (TAR) of SARS-CoV-2-GFP replicon fragments in yeast. Relevant sequences are indicated. CMVp: cytomegalovirus promoter; ORF1ab ΔNSP1: ORF of viral 1ab gene deleted with coding region for NSP1; tGFP-BlaR: GFP-blasticidin deaminase fusion gene; HDVRZ: hepatitis delta virus ribozyme; BGH: bovine growth hormone termination and polyadenylation sequences.