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Verification of a SARS-CoV-2-GFP replicon plasmid. A Recombinant yeast colonies on CSM-Trp agar plates. B Transformed yeast clone was tested for fragments F12–F13, F18–F19, and F23–F25 by yeast colony PCR. C E. coli clones transformed with yeast plasmid were tested for F16–F17 by PCR. D Recombinant SARS-CoV-2-GFP replicon plasmid was tested for all nine sub-genomic fragments used in yeast homologous recombination by PCR.
