Fig. 4.

Inhibitory effect of E64-D and remdesivir on a SARS-CoV-2-GFP replicon. A, B 293 T cells were transfected with SARS-CoV-2-GFP replicon, and ten hours post-transfection, cells were treated or nontreated (BLANK) with E64-D at indicated concentrations. Thirty-six hours post-transfection, cells were observed and photographed by fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm (A) or lysed for Western blot monitored the expression of tGFP-BlaR, actin was used as a loading control (B). C, D 293 T cells were transfected SARS-CoV-2-GFP replicon, and cells were treated or nontreated (BLANK) with remdesivir at indicated concentrations just before transfection. Thirty-six hours post-transfection, cells were observed and photographed using fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm (C), or lysed for Western blot for tGFP-BlaR, Actin was used as a loading control (D).