Fig. 4.

Genetic deletion of Pgrmc1 suppresses the metastasis of breast cancer to the lungs. a H&E staining of the lungs of WT and Pgrmc1 KO mice. Metastatic tumors are shown in deep purple color (scale bar: 800 µm). b Quantification of the number and the area of metastatic tumors per lung lobe. Image J was used for analysis. c Immunostaining of Ki67 in the metastatic tumors of WT and Pgrmc1 KO mice. The number of Ki67-positive cells (pink) were counted. DAPI (blue) was used as an internal control. d Immunostaining of FAK in the metastatic tumors of WT and Pgrmc1 KO mice. FAK-positive areas (pink) were quantified. DAPI (blue) was used as an internal control. Bars in the right panel show the mean ± standard deviation values. *, p < 0.05 versus WT mice