Table 3.
Gene | Primers | Sequence | PCR product size (bp) |
---|---|---|---|
blaKPC |
KPC-F KPC-R |
5′-TGTCACTGTATCGCCGTC-3′ 5′-CTCAGTGCTCTACAGAAAACC-3′ |
1010 |
blaVIM |
VIM-F VIM-R |
5′-CCGATGGTGTTTGGTCGCAT-3′ 5′-GAATGCGCAGCACCAGGAT-3` |
391 |
blaKPC |
KPC-Up KPC-Dw |
5′-GCTACACCTAGCTCCACCTTC-3′ 5′-ACAGTGGTTGGTAATCCATGC-3’ |
968 |
blaVIM |
VIM-Up VIM-Dw |
5’-ATTGGTCTATTTGACCGCGTC-3′ 5′-TGCTACTCAACGACTGAGCG-3’ |
780 |
Primers KPC and VIM -F and -R were used for PCR gene detection
Primers KPC and VIM -Up and -Dw were used for gene sequencing