Fig. 5.

MAPK1–109aa, not circMAPK1, exerts the biological function in GC cells. a MKN45 and BGC823 cells were transfected with empty vector, circMAPK1 vector, ATG mutated circMAPK1 vector and IRES mutated circMAPK1 vector. MAPK1–109aa was determined by immunoblot. b CCK-8 assay was performed to detect proliferation ability of cells mentioned above. c Colony formation assay was performed to detect proliferation ability of cells mentioned above. d The number of cells is counted with image J. e EdU assay was performed to detect proliferation ability of cells mentioned above. Scale bar: 100 μm. f The number of cells is counted with image J. g The effect of four plasmids mentioned above on modulating cell cycle progression was evaluated by flow cytometry assay. h The effect of four plasmids mentioned above on cell migration was examined by Transwell assay. Scale bar: 100 μm. Graph represents mean ± SD; *p < 0.05, **p < 0.01, and ***p < 0.001