Skip to main content
. 2021 Apr 9;20:66. doi: 10.1186/s12943-021-01358-y
RT-PCR primers Forward primer (5′ to 3′) Reverse primer (5′ to 3′) Amplified products (bps)

circMAPK1

(divergent primers)

TCAAGATCTGTGACTTTGGCCT GGTGCTCAAAGGGGCTGATT 187

circMAPK1

(convergent primers)

ACTGCGCTTCAGACATGAGA TGCTGAGGTGTTGTGTCTTCA 143
circ0006203 TCCCCATCACAAGAAGACCTGA TGGTGTAGCCCTTTGGAGTCA 131
circ0008870 CAGCTAACGTTCTGCACCGT GGTGCTCAAAGGGGCTGATT 133
MAPK1 GCACCAACCATCGAGCAAAT CTTGAGGTCACGGTGCAGAA 179
GAPDH GTCAAGGCTGAGAACGGGAA AAATGAGCCCCAGCCTTCTC 158

Full material and methods were described in Additional file 4.