RT-PCR primers | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Amplified products (bps) |
circMAPK1 (divergent primers) |
TCAAGATCTGTGACTTTGGCCT | GGTGCTCAAAGGGGCTGATT | 187 |
circMAPK1 (convergent primers) |
ACTGCGCTTCAGACATGAGA | TGCTGAGGTGTTGTGTCTTCA | 143 |
circ0006203 | TCCCCATCACAAGAAGACCTGA | TGGTGTAGCCCTTTGGAGTCA | 131 |
circ0008870 | CAGCTAACGTTCTGCACCGT | GGTGCTCAAAGGGGCTGATT | 133 |
MAPK1 | GCACCAACCATCGAGCAAAT | CTTGAGGTCACGGTGCAGAA | 179 |
GAPDH | GTCAAGGCTGAGAACGGGAA | AAATGAGCCCCAGCCTTCTC | 158 |
Full material and methods were described in Additional file 4.