Fig. 4.

Circ-MBOAT2 was associated with miR-433-3p in pancreatic cancer cells. a The supposed binding sites between circ-MBOAT2 and miR-433-3p were predicted by circinteractome online database. b The overexpression efficiency of miR-433-3p was determined by qRT-PCR in PANC-1 and SW1990 cells with U6 as an internal reference. c and d The associated relationship between circ-MBOAT2 and miR-433-3p was illustrated by dual-luciferase reporter assay. e and g MiR-433-3p expression was detected by qRT-PCR in 34 pairs of pancreatic cancer and adjacent normal pancreatic tissues as well as HPDE, PANC-1 and SW1990 cells with U6 as an internal reference. f Spearman correlation analysis was employed to assess the linear relationship between circ-MBOAT2 expression and miR-433-3p expression with β-actin/U6 as internal references. h The overexpression efficiency of circ-MBOAT2 was determined by qRT-PCR in PANC-1 and SW1990 cells with β-actin as an internal reference. i The impacts of circ-MBOAT2 silencing and overexpression on miR-433-3p expression were determined by qRT-PCR in PANC-1 and SW1990 cells. **P < 0.01, ***P < 0.001 and ****P < 0.0001