Fig. 7.

MiR-433-3p suppressed pancreatic cancer evolution via binding to GOT1. a The overexpression efficiency of GOT1 was determined by western blot in PANC-1 and SW1990 cells. b The impacts between miR-433-3p mimics and GOT1 overexpression on GOT1 protein expression were revealed by western blot analysis. c-e MTT and cell colony formation assays were employed to illustrate the effects between miR-433-3p mimics and GOT1 overexpression on cell proliferation in PANC-1 and SW1990 cells. f Flow cytometry analysis was used to determine the impacts between miR-433-3p mimics and enforced GOT1 expression on the apoptosis of PANC-1 and SW1990 cells. g and h Transwell invasion and wound-healing assays were performed to investigate the impacts between miR-433-3p mimics and GOT1 overexpression on the invasion and migration of PANC-1 and SW1990 cells. i and j Glutamine and α-KG assay kits were utilized to illustrate the influences between miR-433-3p and GOT1 on glutamine consumption and α-KG production, respectively, in PANC-1 and SW1990 cells. k Glutamate assay kit was used to explain the influences between miR-433-3p and GOT1 on glutamate production. The β-actin was employed for the normalization of GOT1. **P < 0.01, ***P < 0.001 and ****P < 0.0001