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. 2021 Apr 7;16(1):558–569. doi: 10.1515/med-2021-0209

Figure 2.

Figure 2

Effects of circ_0003204 silencing on ox-LDL-induced HUVECs injury. HUVECs were transfected with si-circ_0003204 or si-NC, followed by treatment with ox-LDL. (a) The qPCR was used to detect circ_0003204 expression. (b) CCK8 assay was used to determine the viability of HUVECs. (c) Flow cytometry was performed to test the apoptosis of HUVECs. (d) The activity of caspase-3 was measured using Caspase-3 Activity Assay Kit. (e–g) IL-6, IL-1β, and TNF-α ELISA Assay Kits were used to assess the contents of IL-6, IL-1β, and TNF-α in ox-LDL-induced HUVECs. (h–j) The expression of ROS and MDA and the activity of LDH were evaluated using the ROS, MDA, and LDH Assay Kits, respectively. *P < 0.05.