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. 2021 Apr 7;16(1):558–569. doi: 10.1515/med-2021-0209

Figure 3.

Figure 3

circ_0003204 served as a sponge of miR-330-5p. (a) The fragments of circ_0003204 containing the binding sites or mutant binding sites of miR-330-5p were exhibited. (b and c) Dual-luciferase reporter assay was performed to determine the luciferase activity of circ_0003204 WT or MUT vector in ox-LDL-induced HUVECs. (d) RNA pull-down assay was used to verify the interaction between circ_0003204 and miR-330-5p. The relative expression of circ_0003204 in Bio-miR-330-5p probe or Bio-miR-330-5p MUT probe was detected using qPCR. (e) The miR-330-5p expression in ox-LDL-induced HUVECs or normal HUVECs was assessed by qPCR. (f) HUVECs were transfected with circ_0003204 overexpression plasmid or si-circ_0003204 or their negative controls (pcDNA or si-NC), followed by treatment with ox-LDL. The qPCR was used to test the expression of miR-330-5p. *P < 0.05.